DNeasy Blood & Tissue Kit

DNA isolation / purification Tissue - adipose

Experiment
DNA isolation / purification Tissue - adipose
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

Total DNA was extracted from the adipose tissue using DNeasy Tissue Mini Kit following the manufacturer’s instructions (Qiagen, Hilden, Germany). Extracted DNA was used immediately for PCR using PCR Master Mix (Qiagen) and degenerate primer sets targeting the fiber protein gene.

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Reviews

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - adipose using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
Adenovirus 36 DNA in human adipose tissue.
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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